Back to Search Start Over

Expression of hepatitis B virus core gene products with specific immunoreactivity for e antigen (HBeAg) in Saccharomyces cerevisiae.

Expression of hepatitis B virus core gene products with specific immunoreactivity for e antigen (HBeAg) in Saccharomyces cerevisiae.

Authors :
Bröker M
Noah M
Nassal M
Dietz S
Ochs J
Bäuml O
Waldinger K
Bodenbenner M
Schott U
Grote M
Source :
Journal of biotechnology [J Biotechnol] 1993 Jun; Vol. 29 (3), pp. 243-55.
Publication Year :
1993

Abstract

The e antigen of the hepatitis B virus (HBeAg) was expressed in S. cerevisiae. Yeast-derived HBeAg exhibits high HBe antigenicity while lacking any HBc antigenicity. The production yield of HBeAg expressed in yeast is dependent on the host strains and the nature of the leader sequences used in the plasmid constructions. The recombinant antigen is not secreted into the medium, independent from the leader sequences which are used. A simple extraction procedure was developed, enabling the isolation of HBeAg from the cells without killing them. Recombinant HBeAg derived from yeast can replace plasma-derived antigen in ELISA for determining antibodies to HBeAg.

Details

Language :
English
ISSN :
0168-1656
Volume :
29
Issue :
3
Database :
MEDLINE
Journal :
Journal of biotechnology
Publication Type :
Academic Journal
Accession number :
7763899
Full Text :
https://doi.org/10.1016/0168-1656(93)90056-s