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Expression of hepatitis B virus core gene products with specific immunoreactivity for e antigen (HBeAg) in Saccharomyces cerevisiae.
Expression of hepatitis B virus core gene products with specific immunoreactivity for e antigen (HBeAg) in Saccharomyces cerevisiae.
- Source :
-
Journal of biotechnology [J Biotechnol] 1993 Jun; Vol. 29 (3), pp. 243-55. - Publication Year :
- 1993
-
Abstract
- The e antigen of the hepatitis B virus (HBeAg) was expressed in S. cerevisiae. Yeast-derived HBeAg exhibits high HBe antigenicity while lacking any HBc antigenicity. The production yield of HBeAg expressed in yeast is dependent on the host strains and the nature of the leader sequences used in the plasmid constructions. The recombinant antigen is not secreted into the medium, independent from the leader sequences which are used. A simple extraction procedure was developed, enabling the isolation of HBeAg from the cells without killing them. Recombinant HBeAg derived from yeast can replace plasma-derived antigen in ELISA for determining antibodies to HBeAg.
- Subjects :
- Amino Acid Sequence
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Hepatitis B Core Antigens immunology
Hepatitis B e Antigens isolation & purification
Hepatitis B e Antigens metabolism
Molecular Sequence Data
Plasmids
Protein Sorting Signals metabolism
Recombinant Proteins
Saccharomyces
Schizosaccharomyces
Hepatitis B Core Antigens genetics
Hepatitis B e Antigens immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1656
- Volume :
- 29
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 7763899
- Full Text :
- https://doi.org/10.1016/0168-1656(93)90056-s