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Physiological and biochemical characterization of glyoxalase I, a general marker for cell proliferation, from a soybean cell suspension.
- Source :
-
Planta [Planta] 1993; Vol. 189 (4), pp. 561-6. - Publication Year :
- 1993
-
Abstract
- Using a strictly auxin-dependent soybean (Glycine max (L.) Merr.) cell suspension, we studied the correlation of auxin-dependent cell proliferation and the activity of glyoxalase I (S-lactoylglutathione-lyase EC 4.4.1.5), and enzyme generally associated with cell proliferation in animal, microbial and, as reported recently, also plant systems. We found the activity of glyoxalase I to be modulated during the proliferation cycle, with a maximal activity between day 2 and day 4 of culture growth. After starving the culture of auxins for three subsequent periods, both the enzyme activity and cell-growth could be re-initiated with auxin. Enzyme activity reached its maximum 1 d before cell number was at a maximum. The enzyme was purified to homogeneity and characterized.
Details
- Language :
- English
- ISSN :
- 0032-0935
- Volume :
- 189
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Planta
- Publication Type :
- Academic Journal
- Accession number :
- 7763577
- Full Text :
- https://doi.org/10.1007/BF00198220