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Characterization of fibroblast growth factor receptor 1 RNA expression in the embryonic mouse heart.
- Source :
-
Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 1995 Mar 27; Vol. 752, pp. 406-16. - Publication Year :
- 1995
-
Abstract
- We used reverse transcriptase-polymerase chain reaction (RT-PCR) to clone fibroblast growth factor receptor (FGFR) 1 isoforms from embryonic mouse heart and as a more sensitive method to characterize FGFR1 RNA expression in embryonic and adult mouse hearts. We describe the cloning of both full-length short (2259 base pairs) and long (2526 base pairs) FGFR1 isoform cDNAs which generated 86 and 102 kilodalton proteins, respectively, following in vitro translation. An assessment of FGFR1 RNA indicates that FGFR1-IIIc is the major form in both the embryonic and adult heart but there is an approximately 8.5-fold decrease in RNA levels in the adult. Differential RNA blotting as well as RT-PCR analyses are consistent with a switch in the relative expression of the short versus long FGFR1 isoforms during heart development. The long isoforms are more abundant in the embryo and the short isoforms predominate in the adult. This may be important in the regulation of growth and development of the heart.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Brain metabolism
Cloning, Molecular
Consensus Sequence
DNA Primers
Embryo, Mammalian
Exons
Fibroblast Growth Factor 2 metabolism
Fibroblast Growth Factor 2 physiology
Heart embryology
Mice
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger analysis
RNA, Messenger biosynthesis
Receptor, Fibroblast Growth Factor, Type 1
Restriction Mapping
Sequence Homology, Amino Acid
Gene Expression
Myocardium metabolism
Receptor Protein-Tyrosine Kinases
Receptors, Fibroblast Growth Factor biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0077-8923
- Volume :
- 752
- Database :
- MEDLINE
- Journal :
- Annals of the New York Academy of Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 7755284
- Full Text :
- https://doi.org/10.1111/j.1749-6632.1995.tb17448.x