Effects of lactate dehydrogenase and nicotinamide adenine dinucleotide on human dental plaque pH and acid anion concentrations.

The effect of sucrose rinses supplemented with L-LDH and NAD was investigated. When LDH or NAD were added to the sucrose rinse, the pH fall was less marked than with rinsing with sucrose alone. Significant differences (p < 0.05) in mean minimum pH and cH areas were observed when sucrose rinses were supplemented with LDH, NAD or with a combination of LDH and NAD, when compared with sucrose alone. Plaque fluid concentrations of lactate and acetate significantly decreased with all rinses when compared with sucrose alone. Phosphate levels also decreased, although not significantly, when NAD was the sole supplement. No significant decreases were observed in succinate, formate or propionate concentrations. The results confirm the beneficial effect of LDH in reducing the accumulation of lactate in plaque following a sucrose rinse. The inclusion of NAD in the rinse demonstrated the role of extracellular hydrogen acceptors in the reaction. Further work is required to identify endogenous hydrogen receptors and to clarify the fate of pyruvate resulting from the oxidation of lactate before the potential role of LDH as a cariostatic agent can be fully understood.