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Tissue factor activity of syncytiotrophoblast plasma membranes and tumoral trophoblast cells in culture.
- Source :
-
Thrombosis and haemostasis [Thromb Haemost] 1995 Jan; Vol. 73 (1), pp. 49-54. - Publication Year :
- 1995
-
Abstract
- During pregnancy, important modifications of hemostasis occur resulting in mothers in hypercoagulability and the role of placental cells such as trophoblast cells has been hypothesized. In this study, we first showed that syncytiotrophoblast plasma membranes, isolated from normal human placenta, expressed a strong tissue factor (TF) activity. We then studied TF activity of two continuous trophoblast cell lines (JEG-3 and BeWo) in comparison to human umbilical vein endothelial cells (HUVEC) and transformed human endothelial cells (ECV-304). TF assays were performed on intact detached confluent cells. Unstimulated JEG-3 and BeWo cells exhibited a very high TF activity which slightly increased after 2 to 4 h TNF-alpha stimulation. In contrast, HUVEC and ECV-304 had a lower basal TF activity which was mainly inducible by TNF-alpha, with a maximum effect after 4 to 6 h stimulation. For both cell types, TF activity was decreased to basal value after 16-hour TNF-alpha stimulation. These results support that trophoblast cells are able to express TF but the involvement of this property in the hemostatic physiological changes observed during pregnancy, remains to be demonstrated.
- Subjects :
- Cell Line, Transformed drug effects
Cells, Cultured drug effects
Choriocarcinoma pathology
Cycloheximide pharmacology
Dactinomycin pharmacology
Endothelium, Vascular chemistry
Endothelium, Vascular drug effects
Female
Gene Expression Regulation drug effects
Gene Expression Regulation, Neoplastic drug effects
Humans
Neoplasm Proteins biosynthesis
Neoplasm Proteins genetics
Thromboplastin biosynthesis
Thromboplastin genetics
Tumor Cells, Cultured drug effects
Tumor Necrosis Factor-alpha pharmacology
Umbilical Veins
Uterine Neoplasms pathology
Cell Membrane chemistry
Choriocarcinoma chemistry
Neoplasm Proteins analysis
Pregnancy blood
Thromboplastin analysis
Trophoblasts chemistry
Uterine Neoplasms chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0340-6245
- Volume :
- 73
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Thrombosis and haemostasis
- Publication Type :
- Academic Journal
- Accession number :
- 7740495