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Plasma membrane fatty-acid-binding protein in human placenta: identification and characterization.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1995 Apr 26; Vol. 209 (3), pp. 1011-7. - Publication Year :
- 1995
-
Abstract
- A plasma membrane fatty-acid-binding protein (FABPpm) with a molecular mass of approximately 40 kDa has been identified in human placenta. Binding of both [14C] oleate and [14C] linoleate to human placental membranes was found to be time and temperature dependent. Sulphobromophthalein and alpha-tocopherol did not show competition with the [14C] fatty acid binding. These data suggest that the binding sites are specific for fatty acids. incubation of the membranes with trypsin reduced fatty acid binding activity, indicating that the binding sites were protein in nature. A FABPpm was then solubilized from placental membranes and purified to electrophoretic homogeneity. The fatty acid binding activity of the purified protein was confirmed by autoradioblotting. Polyclonal antiserum raised to FABPpm reduced fatty acid binding to placental membrane significantly compared with preimmune serum. The pI value and the amino acid composition of the protein suggest that the placental FABPpm is different from the previously identified hepatic FABPpm.
- Subjects :
- Amino Acids analysis
Carbon Radioisotopes
Carrier Proteins chemistry
Carrier Proteins isolation & purification
Cell Membrane metabolism
Electrophoresis, Polyacrylamide Gel
Fatty Acid-Binding Protein 7
Fatty Acid-Binding Proteins
Female
Humans
Kinetics
Linoleic Acid
Linoleic Acids metabolism
Liver metabolism
Oleic Acid
Oleic Acids metabolism
Pregnancy
Temperature
Carrier Proteins metabolism
Neoplasm Proteins
Placenta metabolism
Tumor Suppressor Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 209
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 7733952
- Full Text :
- https://doi.org/10.1006/bbrc.1995.1598