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A promoter associated with the neisserial repeat can be used to transcribe the uvrB gene from Neisseria gonorrhoeae.
- Source :
-
Journal of bacteriology [J Bacteriol] 1995 Apr; Vol. 177 (8), pp. 1952-8. - Publication Year :
- 1995
-
Abstract
- A recombinant plasmid capable of restoring UV resistance to an Escherichia coli uvrB mutant was isolated from a genomic library of Neisseria gonorrhoeae. Sequence analysis revealed an open reading frame whose deduced amino acid sequence displayed significant similarity to those of the UvrB proteins of E. coli, Micrococcus luteus, and Streptococcus pneumoniae. A gonococcal uvrB mutant was constructed and found to be extremely sensitive to UV radiation. Transcriptional fusions between portions of the gonococcal uvrB upstream region and a reporter gene were used to localize promoter activity, and the transcriptional start point of the gonococcal uvrB gene was mapped in E. coli by primer extension. A corresponding sigma 70 promoter was identified within a copy of the 26-bp neisserial repeat, and this identification provided the first evidence of a promoter associated with this repetitive element in N. gonorrhoeae.
- Subjects :
- Amino Acid Sequence
Bacterial Proteins genetics
Base Sequence
Chromosome Mapping
Cloning, Molecular
DNA Repair genetics
DNA, Bacterial genetics
Escherichia coli genetics
Genetic Complementation Test
Micrococcus luteus genetics
Molecular Sequence Data
Mutation
Neisseria gonorrhoeae radiation effects
Open Reading Frames
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Streptococcus pneumoniae genetics
Transcription, Genetic
Ultraviolet Rays
DNA Helicases
Escherichia coli Proteins
Genes, Bacterial
Neisseria gonorrhoeae genetics
Promoter Regions, Genetic
Repetitive Sequences, Nucleic Acid
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 177
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 7721686
- Full Text :
- https://doi.org/10.1128/jb.177.8.1952-1958.1995