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[Serodiagnosis of Lyme borreliosis: development and evaluation of a test system for epidemiological studies in the dog].
- Source :
-
DTW. Deutsche tierarztliche Wochenschrift [Dtsch Tierarztl Wochenschr] 1994 Dec; Vol. 101 (12), pp. 476-81. - Publication Year :
- 1994
-
Abstract
- The aim of the study was to develop and validate a serological test system for extended epidemiological investigations to which extend dogs were exposed to Borrelia burgdorferi. For this purpose, 121 samples of dogs which were suspect of an infection and submitted to the laboratory for serological testing, were investigated in an immunofluorescence test (IFT) and to different enzyme-linked immunosorbent assays (ELISA). Valuation of the ELISA systems was assessed in relation to the IFT. Sensitivity, specificity and predictive values were calculated for negative, positive and also borderline values. In the screening test all samples with a positive titre in IFT were judged positive. Samples negative in IFT showed negative results in the screening in only 86%. All samples positive in screening or of borderline value in IFT were again tested using a confirmatory ELISA. By this procedure a specificity of 100% and a sensitivity of 85% was calculated for samples with positive or negative IFT titres, respectively. Samples with IFT borderline titres (1:64 or 1:128) were judged negative in this ELISA in 80% and borderline in 16%. Checking of selected samples in an immunodot test confirmed the ELISA results. Sera being negative in ELISA showed also no specific reaction in this test. When considering the possibility of false positive reactions in IFT, rather high percentages of sensitivity and specificity could be found. Because all real positive samples could be detected using the confirmatory ELISA as a single test, there is no further need in epidemiological investigations to use the screening test. For specific problems the Western blot can be used.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Animals
Dog Diseases epidemiology
Dogs
Enzyme-Linked Immunosorbent Assay veterinary
Evaluation Studies as Topic
Fluorescent Antibody Technique veterinary
Lyme Disease diagnosis
Lyme Disease epidemiology
Antibodies, Bacterial blood
Borrelia burgdorferi Group immunology
Dog Diseases diagnosis
Lyme Disease veterinary
Subjects
Details
- Language :
- German
- ISSN :
- 0341-6593
- Volume :
- 101
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- DTW. Deutsche tierarztliche Wochenschrift
- Publication Type :
- Academic Journal
- Accession number :
- 7720548