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IL-4-based helper activity of CD4+ T cells is radiation sensitive.
- Source :
-
Cellular immunology [Cell Immunol] 1995 Feb; Vol. 160 (2), pp. 248-56. - Publication Year :
- 1995
-
Abstract
- The capacity of CD4+ T cells to induce IgG synthesis in B cells has been known to be radioresistant for more than 20 years. However, the radiation sensitivity of helper T cells with regard to their ability to induce the synthesis of isotypes other than IgG has not been studied. We therefore irradiated KLH-primed lymph node T cells and examined their capacity to induce IgG, IgM, and IgE synthesis in hapten-primed B cells. We demonstrated that while the capacity of KLH-primed lymph node cells to induce IgG synthesis was not affected by irradiation, the capacity of such T cells to induce IgE synthesis was greatly reduced by gamma-irradiation. This was consistent with our observations that IL-4 and IL-5 synthesis in such cells was greatly diminished by irradiation, whereas IL-2 synthesis was only minimally affected. A similar differential sensitivity to irradiation of the helper activity of Th1 and Th2 clones was observed with regard to their ability to induce IgE and IgG synthesis under cognate conditions. Irradiation greatly inhibited the capacity of Th2 clones to induce IgE synthesis, but only minimally affected the capacity of Th1 clones to induce IgG synthesis in primed B cells. The capacity of irradiated Th2 clones to induce IgE synthesis was restored by the addition of IL-4 and IL-5. These results taken together indicated that the sensitivity to irradiation of T helper cells with regard to the induction of IgE but not IgG synthesis was due to the sensitivity to irradiation of the production of IL-4 but not of IL-2.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Animals
CD4-Positive T-Lymphocytes immunology
Conalbumin immunology
Gamma Rays
Gene Expression Regulation
Haptens
Hemocyanins immunology
Immunoglobulin E genetics
Immunoglobulin G biosynthesis
Immunoglobulin G genetics
Interleukin-2 biosynthesis
Interleukin-2 genetics
Interleukin-2 pharmacology
Interleukin-4 biosynthesis
Interleukin-4 genetics
Interleukin-4 pharmacology
Interleukin-5 biosynthesis
Interleukin-5 genetics
Interleukin-5 pharmacology
Lymphocyte Subsets immunology
Mice
Mice, Inbred BALB C
Mice, Inbred DBA
Radiation Tolerance
Recombinant Proteins pharmacology
Th1 Cells immunology
Th1 Cells radiation effects
Th2 Cells immunology
Th2 Cells radiation effects
Trinitrobenzenes immunology
CD4-Positive T-Lymphocytes radiation effects
Immunoglobulin E biosynthesis
Interleukin-4 physiology
Lymphocyte Cooperation radiation effects
Lymphocyte Subsets radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 160
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7720086
- Full Text :
- https://doi.org/10.1016/0008-8749(95)80035-h