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Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases.
- Source :
-
Nature genetics [Nat Genet] 1995 Feb; Vol. 9 (2), pp. 177-83. - Publication Year :
- 1995
-
Abstract
- We have explored the application of the bacteriophage resolvases T4 endonuclease VII and T7 endonuclease I for detecting mutations in genomic DNA. Heteroduplex DNA fragments prepared by amplification from DNA containing known mutations were cleaved by one or both enzymes at nucleotide mismatches created by 3 of 3 short deletions and 13 of 14 point mutations in fragments as large as 940 basepairs. Heteroduplexes representing all four classes of possible single nucleotide mismatches were cleaved, and the sizes of the cleavage products generated correlated with the location of the mutation. We conclude that bacteriophage resolvases may be useful reagents for the rapid screening of DNA for mutations.
- Subjects :
- Bacteriophages enzymology
Base Sequence
DNA Mutational Analysis
Deoxyribonuclease I chemistry
Deoxyribonuclease I isolation & purification
Endodeoxyribonucleases chemistry
Endodeoxyribonucleases isolation & purification
Humans
Methods
Molecular Sequence Data
Mutation
DNA chemistry
DNA metabolism
Deoxyribonuclease I metabolism
Endodeoxyribonucleases metabolism
Nucleic Acid Heteroduplexes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1061-4036
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nature genetics
- Publication Type :
- Academic Journal
- Accession number :
- 7719346
- Full Text :
- https://doi.org/10.1038/ng0295-177