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Inhibition of mammalian protoporphyrinogen oxidase by acifluorfen.
- Source :
-
Biochemistry and molecular biology international [Biochem Mol Biol Int] 1994 Dec; Vol. 34 (6), pp. 1283-9. - Publication Year :
- 1994
-
Abstract
- We have studied the kinetics of the inhibition of mitochondrial protoporphyrinogen oxidase (PPO) from liver and placenta of 3 mammalian species by the diphenyl ether herbicide acifluorfen (AF). AF competitively inhibited PPO from human liver and placenta, mouse liver and pig placenta with respect to its substrate protoporphyrinogen. In contrast, mixed-type inhibition was shown for pig liver. The differing results shown in pig liver may point to structural differences in PPO derived from different species and tissues. We have also compared the effects of AF on the function of PPO in human lymphoblasts from normal subjects and those with variegate porphyria, an inherited disorder of PPO. Competitive inhibition was shown for both and there were no significant differences in the values of Ks or Ki.
- Subjects :
- Animals
Cell Line, Transformed
Flavoproteins
Humans
Kinetics
Lymphocytes enzymology
Mice
Mitochondria drug effects
Mitochondria, Liver drug effects
Mitochondrial Proteins
Oxidoreductases metabolism
Placenta drug effects
Placenta enzymology
Porphyrias, Hepatic enzymology
Protoporphyrinogen Oxidase
Protoporphyrins metabolism
Swine
Mitochondria enzymology
Mitochondria, Liver enzymology
Nitrobenzoates pharmacology
Oxidoreductases antagonists & inhibitors
Oxidoreductases Acting on CH-CH Group Donors
Subjects
Details
- Language :
- English
- ISSN :
- 1039-9712
- Volume :
- 34
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemistry and molecular biology international
- Publication Type :
- Academic Journal
- Accession number :
- 7697001