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Detection of antitrophoblast antibodies in the sera of patients with anticardiolipin antibodies and fetal loss.
- Source :
-
Blood [Blood] 1993 Nov 01; Vol. 82 (9), pp. 2730-41. - Publication Year :
- 1993
-
Abstract
- Women with anticardiolipin antibodies (ACLA) are at increased risk for fetal loss. One potential explanation for this outcome is that sera from these individuals contain antibodies reactive with trophoblast cells, which are involved in the establishment of the uteroplacental vasculature and maintenance of placental blood fluidity. To examine this hypothesis, we compared the incidence of trophoblast-reactive antibodies in 27 patients with ACLA and a history of fetal loss with that in 29 normal pregnant women. Sera from 20 patients, but only one control, contained trophoblast-reactive antibodies (P < .001). These antibodies were not directed against major histocompatibility class I antigens, and reacted with both term and first-trimester trophoblast cells. In most cases, sera from which ACLA were adsorbed by cardiolipin-containing liposomes maintained reactivity against cells. In addition, patient Ig fractions immunoprecipitated an approximately 62-kD protein from the trophoblast cell surface, stimulated the release of arachidonic acid and thromboxane A2 by trophoblasts, and inhibited the binding of prourokinase to trophoblast urokinase receptors. These observations show that sera from women with ACLA and a history of fetal loss contain antitrophoblast antibodies. These antibodies may be serologically distinct from ACLA, and may contribute to the pathogenesis of fetal demise.
- Subjects :
- Antibodies, Antiphospholipid blood
Epitopes
Female
Humans
Immunoglobulin G blood
Immunoglobulin Isotypes blood
In Vitro Techniques
Pregnancy
Recombinant Proteins metabolism
Thromboxane A2 biosynthesis
Trophoblasts physiology
Urokinase-Type Plasminogen Activator metabolism
Abortion, Spontaneous etiology
Antibodies, Anticardiolipin blood
Autoantibodies blood
Trophoblasts immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 82
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 7693045