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Mesangial cells from diabetic NOD mice constitutively secrete increased amounts of insulin-like growth factor-I.
- Source :
-
Endocrinology [Endocrinology] 1993 Oct; Vol. 133 (4), pp. 1783-8. - Publication Year :
- 1993
-
Abstract
- Experimental evidence has suggested that insulin-like growth factor-I (IGF-I) may contribute to diabetic complications. Previously, we and others have shown that normal glomerular mesangial cells have receptors for, synthesize, and exhibit a mitogenic response to IGF-I. We investigated the IGF-I response in cells derived from a genetic model of diabetes, the nonobese diabetic (NOD) mouse. Mesangial cell lines were derived from diabetic (D-NOD) and nondiabetic adult mice. D-NOD cells released more IGF-I into the supernatant and had a decreased binding of IGF-I to surface receptors. Analysis according to Scatchard revealed a decreased number of receptor sites on D-NOD cells, although the structure of the IGF-I receptor visualized by cross-linking was identical for both cell types. Preincubation of D-NOD cells with an antibody to IGF-I resulted in an increase in the number of receptor sites. This suggested that autocrine IGF-I was responsible for the decrease in D-NOD receptor number and that diabetes had resulted in a stable phenotypic change.
- Subjects :
- Animals
Carrier Proteins metabolism
Cell Line
Diabetes Mellitus genetics
Diabetes Mellitus pathology
Female
Glomerular Mesangium pathology
Glucose pharmacology
Insulin-Like Growth Factor Binding Proteins
Mice
Osmolar Concentration
Receptors, Somatomedin metabolism
Diabetes Mellitus metabolism
Glomerular Mesangium metabolism
Insulin-Like Growth Factor I metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0013-7227
- Volume :
- 133
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 7691581
- Full Text :
- https://doi.org/10.1210/endo.133.4.7691581