Back to Search
Start Over
Human IgE mediates stimulus secretion coupling in rat basophilic leukemia cells transfected with the alpha chain of the human high-affinity receptor.
- Source :
-
European journal of immunology [Eur J Immunol] 1993 Jan; Vol. 23 (1), pp. 240-4. - Publication Year :
- 1993
-
Abstract
- Cell surface expression of the alpha chain of the human (h) Fc epsilon receptor type 1 (Fc epsilon R1) can be observed following stable transfection of RBL-2H3 cells with the h alpha chain of the Fc epsilon R1 complex. Association and dissociation constants for hIgE are similar to those previously reported for the ligand and its receptor. The demonstration of mediator release following the cross-linking of hFc epsilon R1 alpha by hIgE and antigen or anti-hFc epsilon R1 alpha antibody suggests that this system will assist the identification of structural motifs and specific amino acids that are involved in the generation of the signal(s) that will initiate and/or propagate the secretion of mediators from mast cells and basophils.
- Subjects :
- Adenosine analogs & derivatives
Adenosine pharmacology
Adenosine-5'-(N-ethylcarboxamide)
Animals
Dexamethasone pharmacology
Gentamicins pharmacology
Histamine Release
Humans
Rats
Receptors, IgE physiology
Serotonin metabolism
Tumor Cells, Cultured
Immunoglobulin E physiology
Leukemia, Basophilic, Acute metabolism
Receptors, IgE genetics
Transfection
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 23
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7678225
- Full Text :
- https://doi.org/10.1002/eji.1830230137