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Tissue-type plasminogen activator-induced invasion and metastasis of murine melanomas.
- Source :
-
Current eye research [Curr Eye Res] 1995 Jun; Vol. 14 (6), pp. 449-58. - Publication Year :
- 1995
-
Abstract
- The role of tissue-type plasminogen activator (tPA) in the 'spontaneous' as well as 'experimental' metastasis of ocular melanomas in mice was evaluated by transfecting the D5.1G4 murine melanoma cell line that possesses low metastatic activity and low tPA activity with a full length cDNA encoding human tPA. For comparison, a highly metastatic melanoma cell line (Queen's) that constitutively expresses high tPA production, was transfected with a cDNA coding for human plasminogen activator inhibitor type 1 (PAI-1). Unlike non-transfected controls, transfected D5.1G4 melanoma cells expressed high levels of tPA and produced extensive pulmonary metastases following intravenous injection. By contrast, PAI-1 transfected Queen's melanoma cells expressed low tPA activity and displayed significantly reduced metastatic potential compared with nontransfected controls. Moreover, PAI-1 transfected Queen's melanoma cells did not metastasize from the eye while nontransfected parental cells produced extensive spontaneous metastases. Expression of tPA activity in transfected and nontransfected cell lines was completely blocked by an anti-tPA antibody. This antibody significantly inhibited the organ localization and frequency of lung metastases of both Queen's and tPA-transfected D5.1G4 melanomas. This study demonstrates that tPA is involved in the metastasis of murine intraocular melanomas.
- Subjects :
- Animals
Anterior Chamber pathology
Female
Gene Expression
Lung Neoplasms metabolism
Lung Neoplasms pathology
Melanoma, Experimental metabolism
Melanoma, Experimental pathology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Nude
Neoplasm Invasiveness
Neoplasm Transplantation
Plasminogen Activator Inhibitor 1 genetics
Plasminogen Activator Inhibitor 1 physiology
Tissue Plasminogen Activator genetics
Tissue Plasminogen Activator immunology
Transfection
Tumor Cells, Cultured
Eye Neoplasms pathology
Lung Neoplasms secondary
Melanoma, Experimental secondary
Tissue Plasminogen Activator physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0271-3683
- Volume :
- 14
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Current eye research
- Publication Type :
- Academic Journal
- Accession number :
- 7671626
- Full Text :
- https://doi.org/10.3109/02713689509003755