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Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase.
- Source :
-
Journal of bacteriology [J Bacteriol] 1995 Sep; Vol. 177 (17), pp. 5148-50. - Publication Year :
- 1995
-
Abstract
- Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.
- Subjects :
- Antibodies, Bacterial
Antibody Specificity
Bacillus subtilis genetics
Bacillus subtilis immunology
Cell Compartmentation
Immunoblotting
Membrane Proteins classification
Membrane Proteins genetics
Membrane Proteins immunology
Membranes enzymology
Molecular Weight
Mutagenesis
Subcellular Fractions enzymology
alpha-Amylases classification
alpha-Amylases genetics
alpha-Amylases immunology
Bacillus subtilis enzymology
Membrane Proteins isolation & purification
alpha-Amylases isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 177
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 7665495
- Full Text :
- https://doi.org/10.1128/jb.177.17.5148-5150.1995