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The measurement of deoxynucleotide (dNTP) pools by radioimmunoassay (RIA).

Authors :
Aherne W
Hardcastle A
Kelland L
Jackman A
Source :
Advances in experimental medicine and biology [Adv Exp Med Biol] 1994; Vol. 370, pp. 801-4.
Publication Year :
1994

Abstract

Radioimmunoassay provides an alternative, sensitive and reproducible high throughput method for the measurement of dNTP pools. The extent and duration of inhibition of TS can be investigated by determination of the TTP and "dUMP" pools and the effects of D1694 and ZD9331 have confirmed their biochemical profiles. The RIAs will be useful in providing information for the design of treatment protocols for TS inhibitors and with the specific assay of dUTP, on mechanisms of cell death in different cell lines.

Subjects

Subjects :
Animals
Biological Transport
Cell Line
Cross Reactions
Deoxyribonucleotides metabolism
Female
Humans
Leukemia L1210 metabolism
Mice
Ovarian Neoplasms
Radioimmunoassay methods
Sensitivity and Specificity
Thymidylate Synthase analysis
Tumor Cells, Cultured
Deoxyribonucleotides analysis
Thymidylate Synthase metabolism

Details

Language :
English
ISSN :
0065-2598
Volume :
370
Database :
MEDLINE
Journal :
Advances in experimental medicine and biology
Publication Type :
Academic Journal
Accession number :
7661027
Full Text :
https://doi.org/10.1007/978-1-4615-2584-4_168
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