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Immunohistochemical localization of pH-sensitive K+ channel, RACTK1.
- Source :
-
The American journal of physiology [Am J Physiol] 1995 Aug; Vol. 269 (2 Pt 1), pp. C496-503. - Publication Year :
- 1995
-
Abstract
- RACTK1 cDNA encodes a pH-sensitive K+ channel from the renal cortical collecting tubule cells, which is only localized in the kidney by Northern blots (Suzuki, M., K. Takahashi, M. Ikeda, H. Hayakawa, A. Ogawa, Y. Kawaguchi, and O. Sakai. Nature Lond. 367: 642-645, 1994). We further investigated the localization of the channel by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemical analysis showed that the signal was detected in various epithelia, including the luminal membrane of the cortical collecting tubules, artery of the kidney, and pancreatic ducts. Interestingly, the signal was also detected in the coronary vascular smooth muscle and cerebral artery. These tissue distributions were confirmed by localization of mRNA determined by RT-PCR. These findings suggest that RACTK1 encoding pH-sensitive K+ channels is widely distributed in epithelia as well as in vascular smooth muscle cells.
- Subjects :
- Animals
Cerebral Arteries metabolism
Coronary Vessels metabolism
Epithelium metabolism
Hydrogen-Ion Concentration
Immunohistochemistry
Kidney Tubules, Collecting
Male
Muscle, Smooth, Vascular metabolism
Pancreatic Ducts metabolism
Polymerase Chain Reaction
Rabbits
Renal Artery metabolism
Tissue Distribution
Transcription, Genetic
Potassium Channels metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 269
- Issue :
- 2 Pt 1
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 7653532
- Full Text :
- https://doi.org/10.1152/ajpcell.1995.269.2.C496