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Cloning and characterization of two cDNA clones encoding seed-specific antimicrobial peptides from Mirabilis jalapa L.
- Source :
-
Plant molecular biology [Plant Mol Biol] 1995 Jul; Vol. 28 (4), pp. 713-21. - Publication Year :
- 1995
-
Abstract
- We have isolated and characterized two cDNA clones (designated MJ1 and MJ2) encoding the two Mirabilis jalapa antimicrobial peptides (Mj-AMP1 and Mj-AMP2, respectively), which were previously purified from seeds of this plant species (Cammue et al. (1992), J Biol Chem 267: 2228-2233). In both cases, the deduced amino acid sequences reveal the presence of a putative signal sequence preceding the mature peptide, indicating that the Mj-AMPs are expressed as preproteins. The Mj-AMP1- and Mj-AMP2-encoding genes are interrupted in their coding sequences by a single intron (380 bp and 900 bp for Mj-AMP1 and Mj-AMP2 genes, respectively). Southern blot analysis indicates that the Mj-AMP-encoding genes belong to a gene family of low complexity. Northern blot analysis suggests seed-specific expression of Mj-AMPs since transcripts of the expected size could only be detected in near-mature and in mature seeds of M. jalapa.
- Subjects :
- Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
Cloning, Molecular
DNA, Complementary genetics
Gene Library
Introns genetics
Molecular Sequence Data
Multigene Family genetics
Plant Diseases
Plants chemistry
Polymerase Chain Reaction
Protein Precursors genetics
Seeds chemistry
Seeds genetics
Selection, Genetic
Tissue Distribution
Anti-Bacterial Agents
Genes, Plant genetics
Peptides
Plant Proteins genetics
Plants genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0167-4412
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 7647302
- Full Text :
- https://doi.org/10.1007/BF00021195