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Detection of amplified Y chromosome-specific sequence by capillary electrophoresis with laser-induced fluorescence.
- Source :
-
Fertility and sterility [Fertil Steril] 1995 Aug; Vol. 64 (2), pp. 447-51. - Publication Year :
- 1995
-
Abstract
- Objective: To develop a sensitive method for genetic diagnosis using capillary electrophoresis with laser-induced fluorescence.<br />Design: Using male DNA diluted with female DNA as an example, ZFY gene from Y chromosome was amplified specifically by polymerase chain reaction (PCR) with fluorescence-labeled primers and detected by capillary electrophoresis with laser-induced fluorescence.<br />Setting: Laser operating laboratory.<br />Participants: Human male and female DNA were extracted from healthy human male and female subjects.<br />Interventions: None.<br />Main Outcome Measures: The concentration of human DNA was determined by using a spectrophotometer at an absorbance of 260 nm.<br />Result: Deoxyribonucleic acid fragments amplified from a single copy of ZFY gene were detected by capillary electrophoresis with laser-induced fluorescence after 35 cycles of PCR amplification.<br />Conclusion: This method is potentially applicable for rapid and sensitive detection of fetal Y chromosome DNA sequence in maternal circulation and of single-cell DNA diagnosis.
Details
- Language :
- English
- ISSN :
- 0015-0282
- Volume :
- 64
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Fertility and sterility
- Publication Type :
- Academic Journal
- Accession number :
- 7615129
- Full Text :
- https://doi.org/10.1016/s0015-0282(16)57751-6