Back to Search
Start Over
Ozone stimulates synthesis of inflammatory cytokines by alveolar macrophages in vitro.
- Source :
-
American journal of respiratory cell and molecular biology [Am J Respir Cell Mol Biol] 1995 Jul; Vol. 13 (1), pp. 60-8. - Publication Year :
- 1995
-
Abstract
- Ozone (O3) is one of the major irritant oxidant gases in photochemical smog. In the present study, the in vitro effect of low concentrations of O3 (0.1 to 1 ppm) was evaluated on cell viability and cytokine secretion by alveolar macrophages (AM) from guinea pigs and healthy subjects. Cell injury was estimated immediately after O3 exposure by evaluation of ATP cell content (measured by bioluminescence) and lactic dehydrogenase (LDH) release in the culture medium. No cytotoxic effect was found: the ATP cell content of both guinea pig AM and human AM did not significantly change after O3 exposure and similarly the LDH release in the culture medium was unchanged. AM-derived cytokines (tumor necrosis factor alpha [TNF alpha], interleukin-1 beta [IL-1 beta], interleukin-6 [IL-6], and interleukin-8 [IL-8]) were evaluated in AM supernatants. O3 exposure was associated with a significant increase in cytokine secretion, with a peak value at 0.4 ppm O3. The exposure of the guinea pig AM to 0.4 ppm O3 for 60 min increased the IL-6 activity by 252 +/- 60% and TNF activity by 202 +/- 35%. The increase in monokine production by the human AM was 443 +/- 208% for TNF alpha, 484 +/- 171% for IL-1 beta, 383 +/- 147% for IL-6, and 226 +/- 45% for IL-8 after a 60-min exposure to 0.4 ppm O3. Lowest O3 concentrations (0.1 and 0.2 ppm) only increased TNF alpha secretion.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Adenosine Triphosphate analysis
Animals
Blotting, Northern
Cell Survival
Cells, Cultured
Cytokines genetics
Female
Guinea Pigs
Humans
Interleukins genetics
Interleukins metabolism
L-Lactate Dehydrogenase analysis
Macrophages, Alveolar pathology
Macrophages, Alveolar ultrastructure
Male
Middle Aged
RNA, Messenger analysis
Smoking
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Cytokines metabolism
Macrophages, Alveolar metabolism
Ozone adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1044-1549
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- American journal of respiratory cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 7598938
- Full Text :
- https://doi.org/10.1165/ajrcmb.13.1.7598938