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Rejection antigen peptides on BALB/c RL male 1 leukemia recognized by cytotoxic T lymphocytes: derivation from the normally untranslated 5' region of the c-akt proto-oncogene activated by long terminal repeat.
- Source :
-
Cancer research [Cancer Res] 1995 Nov 01; Vol. 55 (21), pp. 4780-3. - Publication Year :
- 1995
-
Abstract
- Tumor antigen peptides on BALB/c leukemia RL male 1 that were recognized by cytotoxic T lymphocytes were shown to be derived from a normally untranslated region of the akt proto-oncogene (Uenaka, A. et al., J. Exp. Med., 180: 1599, 1994). We show here that the murine leukemia virus (MuLV) long terminal repeat (LTR) was inserted directly into the exon of c-akt in RL male 1 leukemia and that transcription started from the cap site of the LTR. Translation appeared to start from the ATG codon created in the six nucleotides of unknown origin, which were inserted into the LTR/akt junction. The deduced molecular size is approximately M(r) 59,000 due to the addition of 33 amino acid residues to the normally expressed c-AKT protein. Western blot analysis demonstrated the presence of M(r) 59,000 molecules in an RL male 1 lysate, and their expression at about ten times the level of normal AKT molecules of M(r) 56,000, which is consistent with the increased expression of akt mRNA demonstrated by Northern blot analysis. The findings show that the molecular alteration of AKT protein by insertion of MuLV LTR is the mechanism for creating rejection antigen peptides derived from the untranslated region of akt.
- Subjects :
- Amino Acid Sequence
Animals
Antigens, Neoplasm biosynthesis
Base Sequence
Blotting, Northern
Exons
Gene Expression Regulation, Leukemic
Leukemia Virus, Murine genetics
Male
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Polymorphism, Genetic
Proto-Oncogene Proteins biosynthesis
Proto-Oncogene Proteins c-akt
RNA, Messenger analysis
RNA, Messenger genetics
Antigens, Neoplasm genetics
Antigens, Neoplasm immunology
Leukemia, Experimental immunology
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins genetics
Proto-Oncogene Proteins immunology
Repetitive Sequences, Nucleic Acid physiology
T-Lymphocytes, Cytotoxic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 55
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 7585504