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Molecular cloning and characterization of a TAR-binding nuclear factor from T cells.
- Source :
-
AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 1995 Jun; Vol. 11 (6), pp. 663-9. - Publication Year :
- 1995
-
Abstract
- The TAt protein of the human immunodeficiency virus type 1 (HIV-1) activates the expression of viral mRNA through a cis-acting element in the LTR termed TAR. TAR RNA forms a stable stem-loop structure. Mutagenesis studies indicate that the stem structure, the primary sequence of the loop, and three unpaired bases in the stem (bulge) are important for Tat activation. Using the in vitro-transcribed TAR RNA as a probe, we have cloned a gene (TARBP-b) that encodes a TAR-binding protein from a cDNA expression library derived from Hut-78 cells. Expression of the 1.4-kb TARBP-b mRNA was observed in all mammalian cell lines tested. TARBP-b binds specifically to the bulge region of TAR RNA and trans-activates the HIV-1 long terminal repeat in the presence of ptat and prev expression plasmids. These results suggest that TARBP-b contributes to tat-mediated trans-activation.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cell Line
Gene Expression Regulation, Viral genetics
Gene Products, rev physiology
Gene Products, tat physiology
Genes, Viral genetics
HIV Long Terminal Repeat genetics
Humans
Lymphocytes virology
Mice
Molecular Sequence Data
Nucleic Acid Conformation
RNA Probes
RNA, Messenger biosynthesis
RNA, Viral chemistry
RNA-Binding Proteins chemistry
Sequence Analysis, DNA
rev Gene Products, Human Immunodeficiency Virus
tat Gene Products, Human Immunodeficiency Virus
HIV-1 genetics
RNA, Viral metabolism
RNA-Binding Proteins genetics
T-Lymphocytes virology
Transcriptional Activation genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0889-2229
- Volume :
- 11
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- AIDS research and human retroviruses
- Publication Type :
- Academic Journal
- Accession number :
- 7576925
- Full Text :
- https://doi.org/10.1089/aid.1995.11.663