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Behavior of N-acylated daunorubicins in MDR1 gene transfected and parental cells.
- Source :
-
Biochemical pharmacology [Biochem Pharmacol] 1995 Sep 07; Vol. 50 (6), pp. 889-92. - Publication Year :
- 1995
-
Abstract
- The substrate specificity of the P-glycoprotein (P-170), a multidrug transporter, was studied using N-acylated daunorubicin derivatives and four MDR1 cDNA transfected cell lines. Results showed that N-acetyl-daunorubicin is a substrate, but the longer fatty acid derivatives, N-octanoyl and N-dodecanoyl daunorubicins, are not. This conclusion was reached by flow cytometric drug uptake assay, cell proliferation assays, and confocal microscopy. It was concluded that the longer fatty acid derivatives interact with plasma membranes in a way that affected P-glycoprotein function.
- Subjects :
- 3T3 Cells
ATP Binding Cassette Transporter, Subfamily B, Member 1 antagonists & inhibitors
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
Animals
Cell Division drug effects
Cyclosporine pharmacology
Daunorubicin metabolism
Dose-Response Relationship, Drug
Drug Interactions
Humans
Mice
Transfection
Tumor Cells, Cultured
ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism
Daunorubicin analogs & derivatives
Drug Resistance, Multiple genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-2952
- Volume :
- 50
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemical pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 7575653
- Full Text :
- https://doi.org/10.1016/0006-2952(95)00209-i