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Utilization of activated U937 monocytic cells as a model to evaluate biocompatibility and biodegradation of synthetic calcium phosphate.
- Source :
-
Biomaterials [Biomaterials] 1995 Apr; Vol. 16 (6), pp. 497-503. - Publication Year :
- 1995
-
Abstract
- The use of calcium phosphate biomaterials as a bone substitute necessitates the use of normative biocompatibility and biodegradation techniques which must be fast, simple and reproducible. In the present study, we have developed an in vitro model to study and to compare different calcium phosphate ceramics. After activation with 1,25-dihydroxy-vitamin D3 and phorbol 12,13-dibutyrate, the monoblastic U937 cells became multinucleated, expressed tartrate-resistant acid phosphatase and several markers of monocyte/macrophage differentiation. Activated U937 cells did not express the vitronectin receptor (VNR) (as revealed using monoclonal antibodies 23C6 or 13C2) but around 25% of the cells were strongly reactive with 211D, a novel monoclonal antibody that recognizes an osteoclast-specific membrane antigenic determinant. These cells remain active/viable with hydroxyapatite (HA) or beta-tricalcium phosphate (beta-TCP) ceramics. In conclusion, activated U937 cells are good candidates to use in a normative in vitro method to evaluate new biomaterials.
- Subjects :
- Acid Phosphatase metabolism
Antibodies, Monoclonal
Antibody Specificity
Biocompatible Materials standards
Biodegradation, Environmental
Calcium Phosphates chemistry
Ceramics
Dentin metabolism
Flow Cytometry
Giant Cells cytology
Giant Cells enzymology
Giant Cells metabolism
Humans
Integrins metabolism
Leukemia, Myeloid
Macrophages cytology
Macrophages metabolism
Microscopy, Electron, Scanning
Receptors, Cytoadhesin metabolism
Receptors, Vitronectin
Reproducibility of Results
Staining and Labeling
Tumor Cells, Cultured
Calcitriol pharmacology
Calcium Phosphates metabolism
Monocytes metabolism
Phorbol 12,13-Dibutyrate pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0142-9612
- Volume :
- 16
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 7544631
- Full Text :
- https://doi.org/10.1016/0142-9612(95)98824-x