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Detection of autoantibodies against bullous pemphigoid and pemphigus antigens by an enzyme-linked immunosorbent assay using the bacterial recombinant proteins.
- Source :
-
Experimental dermatology [Exp Dermatol] 1995 Apr; Vol. 4 (2), pp. 112-6. - Publication Year :
- 1995
-
Abstract
- To develop a new method to evaluate autoantibodies in various autoimmune bullous skin diseases, we examined reactivity of bullous pemphigoid (BP) and pemphigus vulgaris (PV) patients' sera with partial bacterial fusion proteins of the 230 kD BP antigen (BPAG1) and PV antigen, respectively, by an enzyme-linked immunosorbent assay (ELISA), and compared the results with those of immunoblotting. We used two fusion proteins derived from the mouse BPAG1 and a fusion protein derived from the amino-terminus (EC1-2) of PV antigen. For both BP and PV sera, the ELISA scores were well correlated with the reactivities on immunoblot assays. The present study indicates that ELISA using recombinant antigen proteins in various autoimmune bullous skin diseases will be a new useful technique to detect the autoantibodies. However, development of recombinant proteins with the entire molecule and correct conformation will be necessary to establish a perfect ELISA system in the future.
- Subjects :
- Autoantibodies immunology
Autoantigens genetics
Autoimmune Diseases blood
Blotting, Western
Cadherins genetics
Desmoglein 3
Dystonin
Epitopes genetics
Fluorescent Antibody Technique
Humans
Pemphigoid, Bullous blood
Pemphigus blood
Peptide Fragments genetics
Sensitivity and Specificity
Collagen Type XVII
Autoantibodies blood
Autoantigens immunology
Autoimmune Diseases immunology
Cadherins immunology
Carrier Proteins
Collagen
Cytoskeletal Proteins
Enzyme-Linked Immunosorbent Assay
Epitopes immunology
Nerve Tissue Proteins
Non-Fibrillar Collagens
Pemphigoid, Bullous immunology
Pemphigus immunology
Peptide Fragments immunology
Recombinant Fusion Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0906-6705
- Volume :
- 4
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 7543794
- Full Text :
- https://doi.org/10.1111/j.1600-0625.1995.tb00232.x