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Hyaluronan binding by CD44 is regulated by a phosphorylation-independent mechanism.
- Source :
-
European journal of immunology [Eur J Immunol] 1995 Jul; Vol. 25 (7), pp. 1883-7. - Publication Year :
- 1995
-
Abstract
- CD44 is an adhesion receptor for which the major characterized ligand is the extracellular matrix glycosaminoglycan, hyaluronan. This interaction underlies CD44-mediated cell attachment, cell migration, and matrix remodelling during development and wound healing. Truncation of the CD44 cytoplasmic domain does not prevent cell surface expression of this hyaluronan receptor but it dramatically impairs ligand binding. In this study we have examined the role of phosphorylation in regulating this function by mutating the target serine residues to either neutral amino acids with the aim of creating a phosphorylation-incompetent molecule, or to acidic residues to mimic a fully phosphorylated CD44. In transfected AKR1 cells the behavior of both the neutral and acidic mutants was indistinguishable from wild-type CD44, indicating that there is a phosphorylation-independent mechanism involved in regulating hyaluronan binding.
- Subjects :
- Amino Acid Sequence
Base Sequence
Cell Adhesion
DNA Primers chemistry
Humans
Hyaluronan Receptors
In Vitro Techniques
Molecular Sequence Data
Mutagenesis, Site-Directed
Phosphorylation
Sequence Deletion
Structure-Activity Relationship
Carrier Proteins metabolism
Cell Adhesion Molecules metabolism
Hyaluronic Acid metabolism
Receptors, Cell Surface metabolism
Receptors, Lymphocyte Homing metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 25
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7542594
- Full Text :
- https://doi.org/10.1002/eji.1830250714