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Full-length but not truncated CD34 inhibits hematopoietic cell differentiation of M1 cells.
- Source :
-
Blood [Blood] 1995 Jun 01; Vol. 85 (11), pp. 3040-7. - Publication Year :
- 1995
-
Abstract
- CD34 is expressed on human and murine hematopoietic stem and progenitor cells and its clinical usefulness for isolation of stem/progenitor cells has been well established. Although expression of CD34 is regulated in a developmental stage-specific manner, the function of CD34 is not known. Recently we have shown that both a full-length and truncated form of CD34 protein is expressed by hematopoietic cells (Blood 84:691, 1994). To test whether failure to suppress either form of CD34 could affect terminal myeloid differentiation, we constitutively expressed these CD34 proteins in murine M1 myeloid leukemia cells, which can be terminally differentiated to macrophages by treatment with interleukin-6 of leukemia inhibitory factor. Surprisingly our results show that forced expression of the full-length but not the truncated form of CD34 impedes terminal differentiation by these agents. Because the difference between the two forms of CD34 protein resides in the length of their respective cytoplasmic tail domains, our findings strongly suggest that the cytoplasmic domain region of full-length CD34 is responsible for the observed maturation arrest phenotype. These findings suggest a potential negative regulatory role for full-length CD34 in hematopoietic cell differentiation and may explain, at least in part, the block in maturation observed in CD34+ acute myeloid leukemia.
- Subjects :
- Acute Disease
Animals
Antigens, CD chemistry
Antigens, CD genetics
Antigens, CD34
Base Sequence
Cell Differentiation drug effects
Gene Expression Regulation, Leukemic drug effects
Growth Inhibitors pharmacology
Interleukin-6 pharmacology
Leukemia Inhibitory Factor
Lymphokines pharmacology
Macrophages
Mice
Molecular Sequence Data
Peptide Fragments genetics
Phagocytosis
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins genetics
Tumor Cells, Cultured drug effects
Antigens, CD physiology
Hematopoietic Stem Cells drug effects
Leukemia, Myeloid pathology
Recombinant Fusion Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 85
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 7538813