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Exogenous expression of human granulocyte colony-stimulating factor receptor in a B-lineage acute lymphoblastic leukemia cell line: a possible model for mixed lineage leukemia.
- Source :
-
Leukemia research [Leuk Res] 1995 Apr; Vol. 19 (4), pp. 249-56. - Publication Year :
- 1995
-
Abstract
- We report evidence that the granulocyte colony-stimulating factor (G-CSF) receptor is present and may be functioning on blast cells from some patients with myeloid surface antigen positive (My+) acute lymphoblastic leukemia (ALL). In the present study, a human G-CSF receptor expression plasmid was transfected into a newly established B-lineage ALL cell line 'Tanoue' and its subclone 'ST' by lipofection to investigate whether expression of the G-CSF receptor and G-CSF stimulation would induce myeloid characteristics on myeloid surface antigen negative (My-) ALL cells. The G-CSF receptor became detectable on the transfected cells (GR-Tanoue and GR-ST), with dissociation constant values of 50-130 pmol/l, and maximal binding sites (Bmax of 77-6100 sites/cell on receptor binding assays. Short term culture with recombinant human G-CSF induced myeloid differentiation (a two to three-fold increase in CD33 and CD15 expression), and a moderate 3H-thymidine uptake (stimulation index, 1.75) only in the GR-ST clone no. 15 which expressed a high number of G-CSF receptors (Bmax, 6100 sites/cell). Our data show that (a) exogenous expression of the G-CSF receptor and G-CSF stimulation can induce myeloid characteristics on ALL cells; and (b) in the G-CSF receptor-expressing cells, there is a correlation between the number of G-CSF receptors and cell responsiveness to G-CSF in either proliferation or differentiation.
- Subjects :
- Burkitt Lymphoma immunology
Burkitt Lymphoma pathology
Cell Division drug effects
Clone Cells
Granulocyte Colony-Stimulating Factor pharmacology
Humans
Immunophenotyping
In Vitro Techniques
Transfection
Tumor Cells, Cultured
Burkitt Lymphoma metabolism
Receptors, Granulocyte Colony-Stimulating Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0145-2126
- Volume :
- 19
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Leukemia research
- Publication Type :
- Academic Journal
- Accession number :
- 7538617
- Full Text :
- https://doi.org/10.1016/0145-2126(94)00156-5