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Further characterization of the 5'-flanking region of the rat lactase-phlorizin hydrolase gene.

Further characterization of the 5'-flanking region of the rat lactase-phlorizin hydrolase gene.

Authors :
Verhave M
Krasinski SD
Maas SM
Smiers FJ
Mishra K
Breeuwsma NG
Naim HY
Grand RJ
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1995 Apr 26; Vol. 209 (3), pp. 989-95.
Publication Year :
1995

Abstract

The lactase-phlorizin hydrolase gene is widely used as a marker of intestinal differentiation. Recent evidence demonstrating that transcription plays a major role in the regulation of this gene suggests that study of the 5'-flanking region will allow an understanding of how the expression of this gene is controlled. However, sequence, restriction, and primer extension analysis of a rat genomic clone has revealed that previously published data are incomplete. In the present study, we used a directed sequencing strategy to carefully analyze this region. Our expanded analysis of the 5'-flanking region of the lactase-phlorizin hydrolase gene should facilitate future studies of its structure and function.

Details

Language :
English
ISSN :
0006-291X
Volume :
209
Issue :
3
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
7537502
Full Text :
https://doi.org/10.1006/bbrc.1995.1595