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Interaction of primer tRNA(Lys3) with the p51 subunit of human immunodeficiency virus type 1 reverse transcriptase: a possible role in enzyme activation.

Authors :
Zakharova OD
Tarrago-Litvak L
Fournier M
Andreola ML
Repkova MN
Venyaminova AG
Litvak S
Nevinsky GA
Source :
FEBS letters [FEBS Lett] 1995 Mar 20; Vol. 361 (2-3), pp. 287-90.
Publication Year :
1995

Abstract

In the interaction between HIV-1 RT and tRNA(Lys3) each subunit of the heterodimer interacts with tRNA showing a different affinity: Kd (p66) = 23 nM, Kd (p51) = 140 nM. Preincubation of heterodimeric RT with tRNA, at concentrations similar to that of the Kd value for p51, leads to an increase of the catalytic activity on poly(A)-oligo(dT). These results were compared to those using different tRNA analogs: oxidized tRNA, tRNAs lacking one, two or three nucleotides from the 3'-end, or ribo- and deoxyribonucleotides mimicking the anticodon loop sequence. In all cases, tRNA analogs were weaker activators of HIV-1 RT than natural tRNA. A possible mechanism of RT p66/p51 activation by tRNA and its analogs, mediated through the p51 subunit, is discussed.

Details

Language :
English
ISSN :
0014-5793
Volume :
361
Issue :
2-3
Database :
MEDLINE
Journal :
FEBS letters
Publication Type :
Academic Journal
Accession number :
7535248
Full Text :
https://doi.org/10.1016/0014-5793(95)00200-s