Back to Search
Start Over
Interaction of primer tRNA(Lys3) with the p51 subunit of human immunodeficiency virus type 1 reverse transcriptase: a possible role in enzyme activation.
- Source :
-
FEBS letters [FEBS Lett] 1995 Mar 20; Vol. 361 (2-3), pp. 287-90. - Publication Year :
- 1995
-
Abstract
- In the interaction between HIV-1 RT and tRNA(Lys3) each subunit of the heterodimer interacts with tRNA showing a different affinity: Kd (p66) = 23 nM, Kd (p51) = 140 nM. Preincubation of heterodimeric RT with tRNA, at concentrations similar to that of the Kd value for p51, leads to an increase of the catalytic activity on poly(A)-oligo(dT). These results were compared to those using different tRNA analogs: oxidized tRNA, tRNAs lacking one, two or three nucleotides from the 3'-end, or ribo- and deoxyribonucleotides mimicking the anticodon loop sequence. In all cases, tRNA analogs were weaker activators of HIV-1 RT than natural tRNA. A possible mechanism of RT p66/p51 activation by tRNA and its analogs, mediated through the p51 subunit, is discussed.
- Subjects :
- Anticodon
Base Sequence
Enzyme Activation
HIV Reverse Transcriptase
HIV-1 enzymology
Kinetics
Macromolecular Substances
Molecular Sequence Data
Protein Binding
RNA, Transfer, Amino Acyl chemistry
RNA-Directed DNA Polymerase chemistry
Spectrometry, Fluorescence
Structure-Activity Relationship
Time Factors
RNA, Transfer, Amino Acyl metabolism
RNA-Directed DNA Polymerase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 361
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 7535248
- Full Text :
- https://doi.org/10.1016/0014-5793(95)00200-s