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Release of fibroblast growth factor-1 by human squamous cell carcinoma correlates with autocrine cell growth.
- Source :
-
In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 1994 Nov; Vol. 30A (11), pp. 790-5. - Publication Year :
- 1994
-
Abstract
- A squamous cell carcinoma cell line Nakata proliferated in serum-free culture and was not responsive to exogenous fibroblast growth factor-1 (FGF-1). Immunostaining revealed that Nakata cells expressed FGF-1 in their cytoplasms and nuclei. Two molecular mass species of FGF-1 (16 and 18 kDa) were identified in cell extracts by Western blot. These cells also expressed high-affinity FGF-1 binding sites (Kd = 360 pM, 28,000 sites/cell). The results of cross-linking with [125I]FGF-1 demonstrated the presence of two bands with molecular masses of 160 and 140 kDa. The addition of FGF-1 specific antisense oligonucleotides at 25 microM to Nakata cells resulted in an 82% inhibition in cell growth and suppressed FGF-1 expression. This effect was dose-dependent and specific, because sense oligonucleotides were ineffective in inhibiting cell growth. In addition, Nakata cell growth was suppressed by an anti-FGF-1 neutralizing antibody, which resulted in a 52% inhibition at 8 micrograms/ml. These results demonstrate that Nakata cells produce FGF-1, and indicate that this growth factor acts in an autocrine manner by interacting with FGF-1 binding sites on Nakata cells.
- Subjects :
- Base Sequence
Blotting, Western
Carcinoma, Squamous Cell genetics
Cell Division physiology
Culture Media, Serum-Free
DNA Primers analysis
DNA Primers chemistry
DNA Primers genetics
DNA, Neoplasm analysis
DNA, Neoplasm chemistry
DNA, Neoplasm genetics
Fibroblast Growth Factor 1 genetics
Fibroblast Growth Factor 1 physiology
Gene Expression Regulation, Neoplastic
Humans
Molecular Sequence Data
Mouth Neoplasms genetics
Oligonucleotides, Antisense analysis
Oligonucleotides, Antisense chemistry
Oligonucleotides, Antisense genetics
Tumor Cells, Cultured
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Fibroblast Growth Factor 1 metabolism
Mouth Neoplasms metabolism
Mouth Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1071-2690
- Volume :
- 30A
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- In vitro cellular & developmental biology. Animal
- Publication Type :
- Academic Journal
- Accession number :
- 7533598
- Full Text :
- https://doi.org/10.1007/BF02631303