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[Nomographs for determination of sedimentation constants of nucleic acids by means of centrifugation in sucrose gradients using the "Sinco" ultracentrifuge].

Authors :
Osterman LA
Source :
Molekuliarnaia biologiia [Mol Biol (Mosk)] 1977 May-Jun; Vol. 11 (3), pp. 694-705.
Publication Year :
1977

Abstract

A set of nomographs for determination of standard sedimentation constant values for DNA and RNA directly from the results of centrifugation without any marker is calculated and plotted for 9 Spinco bucket-rotors, 5 temperatures, and two commonly used sucrose gradients (5--20 and 15--30%).

Details

Language :
Russian
ISSN :
0026-8984
Volume :
11
Issue :
3
Database :
MEDLINE
Journal :
Molekuliarnaia biologiia
Publication Type :
Academic Journal
Accession number :
752798