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Molecular cloning of cDNA encoding human DNA helicase Q1 which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1994 Nov 11; Vol. 22 (22), pp. 4566-73. - Publication Year :
- 1994
-
Abstract
- A complementary DNA encoding DNA-dependent ATPase Q1 possessing DNA helicase activity, which is the major DNA-dependent ATPase in human cell extracts, was cloned from a cDNA library of human KB cells. The predicted amino acid sequence has seven consecutive motifs conserved in the RNA and DNA helicase super family and DNA helicase Q1 belongs to DEXH helicase family. A homology search indicated that helicase Q1 had 47% homology in the seven conserved regions with Escherichia coli RecQ protein. Three RNA bands of 4.0, 3.3, and 2.2 kilobases were detected in HeLa cells by Northern blotting. Analysis of the genomic DNA indicated the presence of a homologous gene in mouse cells. The DNA helicase Q1 gene was localized on the short arm of human chromosome 12 at 12p12.
- Subjects :
- Adenosine Triphosphatases chemistry
Adenosine Triphosphatases isolation & purification
Adenosine Triphosphatases metabolism
Amino Acid Sequence
Animals
Base Sequence
Cells, Cultured
Chromosome Mapping
Cloning, Molecular
DNA Helicases chemistry
DNA Helicases isolation & purification
DNA Helicases metabolism
Escherichia coli genetics
Gene Library
HeLa Cells chemistry
Humans
KB Cells
Mice
Molecular Sequence Data
RNA analysis
RecQ Helicases
Sequence Analysis
Sequence Analysis, DNA
Adenosine Triphosphatases genetics
Chromosomes, Human, Pair 12
DNA Helicases genetics
DNA, Complementary genetics
Sequence Homology, Amino Acid
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 22
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 7527136
- Full Text :
- https://doi.org/10.1093/nar/22.22.4566