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Damage and mutagenesis of E. coli and bacteriophage lambda induced by oxathiolane and aziridinyl steroids.

Authors :
Qadri SA
Ahmad M
Source :
Mutation research [Mutat Res] 1994 Dec 01; Vol. 311 (2), pp. 199-208.
Publication Year :
1994

Abstract

lambda-Escherichia coli complexes exhibited remarkable sensitivity to the treatment with test steroidal derivatives in the presence of Cu(II). The decline in plaque-forming units after steroid treatment was more pronounced in complexes with some of the radiation repair-defective mutants of E. coli K-12, i.e., recA, lexA and polA, as compared to uvrA and wild-type strains. The red gene of lambda phage and recA gene of E. coli seem to have a complementary effect on the steroid-induced lesions. An enhanced level of mutagenesis was observed when steroid-treated E. coli cells were transformed with steroid-treated pBR322 plasmid DNA. A remarkable degree of c mutation was also observed when steroid I-treated phage particles were allowed to adsorb on steroid-treated wild-type bacteria. Moreover, the oxathiolane steroid treatment of lambda cI857-E. coli lysogen resulted in prophage induction in nutrient broth even at 32 degrees C. Thus on the basis of these results, the role of SOS repair system in steroid-induced mutagenesis and repair of DNA lesions in E. coli and bacteriophage lambda has been suggested.

Details

Language :
English
ISSN :
0027-5107
Volume :
311
Issue :
2
Database :
MEDLINE
Journal :
Mutation research
Publication Type :
Academic Journal
Accession number :
7526184
Full Text :
https://doi.org/10.1016/0027-5107(94)90177-5