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Damage and mutagenesis of E. coli and bacteriophage lambda induced by oxathiolane and aziridinyl steroids.
- Source :
-
Mutation research [Mutat Res] 1994 Dec 01; Vol. 311 (2), pp. 199-208. - Publication Year :
- 1994
-
Abstract
- lambda-Escherichia coli complexes exhibited remarkable sensitivity to the treatment with test steroidal derivatives in the presence of Cu(II). The decline in plaque-forming units after steroid treatment was more pronounced in complexes with some of the radiation repair-defective mutants of E. coli K-12, i.e., recA, lexA and polA, as compared to uvrA and wild-type strains. The red gene of lambda phage and recA gene of E. coli seem to have a complementary effect on the steroid-induced lesions. An enhanced level of mutagenesis was observed when steroid-treated E. coli cells were transformed with steroid-treated pBR322 plasmid DNA. A remarkable degree of c mutation was also observed when steroid I-treated phage particles were allowed to adsorb on steroid-treated wild-type bacteria. Moreover, the oxathiolane steroid treatment of lambda cI857-E. coli lysogen resulted in prophage induction in nutrient broth even at 32 degrees C. Thus on the basis of these results, the role of SOS repair system in steroid-induced mutagenesis and repair of DNA lesions in E. coli and bacteriophage lambda has been suggested.
- Subjects :
- Aziridines toxicity
Bacteriophage lambda genetics
Cholestanes toxicity
Cholestanols toxicity
DNA Damage
Escherichia coli genetics
Free Radical Scavengers metabolism
Reactive Oxygen Species metabolism
Thiones toxicity
Virus Activation
Bacteriophage lambda drug effects
Escherichia coli drug effects
Mutagenesis
Mutagens toxicity
SOS Response, Genetics
Steroids toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0027-5107
- Volume :
- 311
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Mutation research
- Publication Type :
- Academic Journal
- Accession number :
- 7526184
- Full Text :
- https://doi.org/10.1016/0027-5107(94)90177-5