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Regulation of adhesion of CD4+ T lymphocytes to intact or heparinase-treated subendothelial extracellular matrix by diffusible or anchored RANTES and MIP-1 beta.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1994 Dec 01; Vol. 153 (11), pp. 4899-906. - Publication Year :
- 1994
-
Abstract
- Chemokines, a superfamily of 8- to 11-kDa mediators of inflammation, affect the attachment of immune cells to vascular endothelia by binding to cell surface glycosaminoglycans. We analyzed whether chemokines are also involved in interactions between CD4+ T lymphocytes and the subendothelial extracellular matrix (ECM). Soluble mediators, such as MIP-1 beta and RANTES, induced the binding of resting human CD4+ T cells to ECM in an integrin-dependent manner. Both MIP-1 beta and RANTES bound to intact ECM and retained their adhesive properties, and moreover, ECM-bound RANTES and MIP-1 beta prolonged the time course of interactions between the CD4+ T cells and the ECM. Because the adhesive effect of these chemokines was restricted by an inhibitor of GTP-binding proteins, the adhesive effect of ECM-bound RANTES and MIP-1 beta, which requires an intact cytoskeleton, seems to involve activation of a G protein-linked receptor. MIP-1 beta and RANTES exert their pro-adhesive effects through interactions with glycosaminoglycans, because heparinase-treated ECM did not bound chemokines and because the chemokines ability to induce T cell adhesion was abrogated if: 1) either of the chemokines is pretreaed with heparin or heparan-sulfate (HS), 2) HS is removed from intact ECM by heparinase, an HS-specific endoglycosidase, or 3) the ECM-bound chemokines are released by pretreatment with heparinase. Hence, the adhesive effects of immobilized chemokines is not restricted to T cells interacting with endothelial cells, but also affects the migration of immune cells which reside and function in the context of ECM.
- Subjects :
- Cells, Cultured
Chemokine CCL4
Chemokine CCL5
Cytokines metabolism
Endothelium, Vascular immunology
Heparin Lyase
Humans
Lymphokines metabolism
Macrophage Inflammatory Proteins
Monokines metabolism
Polysaccharide-Lyases physiology
Protein Binding immunology
CD4-Positive T-Lymphocytes physiology
Cell Adhesion immunology
Cytokines physiology
Extracellular Matrix immunology
Lymphokines physiology
Monokines physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 153
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 7525718