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Morphological properties and projections of electrophysiologically characterized neurons in the guinea-pig submucosal plexus.
- Source :
-
Neuroscience [Neuroscience] 1994 Apr; Vol. 59 (4), pp. 1093-110. - Publication Year :
- 1994
-
Abstract
- Intracellular recordings were made from 73 guinea-pig submucosal neurons using neurobiotin-filled microelectrodes; subsequently, neuropeptide immunoreactivity, morphology and nerve fibre projections were determined. Five distinct groups of cells could be distinguished: S cells with inhibitory input (61%), S cells without inhibitory input (19%), AH cells (8%), S-AH cells (5%), and glial networks. S cells with inhibitory input were immunoreactive for vasoactive intestinal polypeptide and showed Dogiel Type III morphology with the axon branching and coursing through two to 12 ganglia; varicosities and tufts of varicosities were observed surrounding other cell bodies as well as over blood vessels. S cells without inhibitory input primarily were immunoreactive for neuropeptide Y; they also showed Dogiel Type III morphology and similar, though shorter, axonal projections and varicose features surrounding other neurons. AH cells, which most likely contained substance P, lacked synaptic input and exhibited Dogiel Type II morphology; they branched more extensively than S cells and also formed varicose tufts within other ganglia. S-AH cells combined electrophysiological properties of S cells with inhibitory input and AH cells and did not show consistent morphological or histochemical characteristics. Typical glial networks were observed; in addition, on two occasions unusual networks of dye and electrical coupling between S cells without inhibitory input and a glial complex were observed. These results suggest that vasoactive intestinal polypeptide-containing S cells may act as interneurons which mediate a slow excitatory synaptic potential; that neuropeptide Y-containing S cells, which are known to be cholinergic, may play a role as cholinergic interneurons mediating the nicotinic fast excitatory synaptic potential; and that AH neurons also may provide cholinergic innervation to other submucosal neurons in addition to their previously described dual projections into mucosa and myenteric plexus.
- Subjects :
- Action Potentials
Animals
Electrophysiology
Ganglia, Autonomic ultrastructure
Guinea Pigs
Interneurons ultrastructure
Intestine, Small innervation
Neuropeptide Y analysis
Receptors, Nicotinic physiology
Submucous Plexus physiology
Substance P analysis
Vasoactive Intestinal Peptide analysis
Neurons ultrastructure
Submucous Plexus cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 59
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 7520133
- Full Text :
- https://doi.org/10.1016/0306-4522(94)90308-5