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ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons.
- Source :
-
The Journal of cell biology [J Cell Biol] 1994 Jul; Vol. 126 (2), pp. 391-401. - Publication Year :
- 1994
-
Abstract
- The ERM family members, ezrin, radixin, and moesin, localizing just beneath the plasma membranes, are thought to be involved in the actin filament/plasma membrane association. To identify the integral membrane protein directly associated with ERM family members, we performed immunoprecipitation studies using antimoesin mAb and cultured baby hamster kidney (BHK) cells metabolically labeled with [35S]methionine or surface-labeled with biotin. The results indicated that moesin is directly associated with a 140-kD integral membrane protein. Using BHK cells as antigens, we obtained a mAb that recognized the 140-kD membrane protein. We next cloned a cDNA encoding the 140-kD membrane protein and identified it as CD44, a broadly distributed cell surface glycoprotein. Immunoprecipitation with various anti-CD44 mAbs showed that ezrin and radixin, as well as moesin, are associated with CD44, not only in BHK cells, but also in mouse L fibroblasts. Furthermore, immunofluorescence microscopy revealed that in both BHK and L cells, the Triton X-100-insoluble CD44 is precisely colocalized with ERM family members. We concluded that ERM family members work as molecular linkers between the cytoplasmic domain of CD44 and actin-based cytoskeletons.
- Subjects :
- Actins metabolism
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Blood Proteins analysis
Carrier Proteins genetics
Carrier Proteins immunology
Carrier Proteins isolation & purification
Cell Line
Cricetinae
Cytoskeleton chemistry
DNA, Complementary analysis
Epitopes genetics
Epitopes immunology
Hyaluronan Receptors
Membrane Proteins analysis
Mice
Molecular Sequence Data
Phosphoproteins analysis
Precipitin Tests
Proteins analysis
Rabies virus chemistry
Rabies virus immunology
Receptors, Cell Surface genetics
Receptors, Cell Surface immunology
Receptors, Cell Surface isolation & purification
Receptors, Lymphocyte Homing genetics
Receptors, Lymphocyte Homing immunology
Receptors, Lymphocyte Homing isolation & purification
Recombinant Fusion Proteins biosynthesis
Recombinant Fusion Proteins immunology
Sequence Analysis, DNA
Blood Proteins metabolism
Carrier Proteins metabolism
Cytoskeletal Proteins
Cytoskeleton metabolism
Membrane Proteins metabolism
Microfilament Proteins
Phosphoproteins metabolism
Proteins metabolism
Receptors, Cell Surface metabolism
Receptors, Lymphocyte Homing metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 126
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 7518464
- Full Text :
- https://doi.org/10.1083/jcb.126.2.391