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Human platelet antigen-1 (Zw) typing of fetuses by analysis of polymerase chain reaction-amplified genomic DNA from amniocytes.
- Source :
-
Transfusion medicine (Oxford, England) [Transfus Med] 1994 Mar; Vol. 4 (1), pp. 15-9. - Publication Year :
- 1994
-
Abstract
- Prenatal typing for the human platelet antigens-1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA-1 incompatibility in which the father is heterozygous for the HPA-1a antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele-specific restriction enzyme analysis on polymerase chain reaction (PCR)-amplified DNA purified from amniocytes. This assays allows early second trimester typing for HPA-1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.
- Subjects :
- Alleles
Amniocentesis
Antigens, Human Platelet genetics
Cells, Cultured
Cordocentesis
DNA isolation & purification
Deoxyribonuclease HpaII
Deoxyribonucleases, Type II Site-Specific
Female
Humans
Immunization
Integrin beta3
Male
Pedigree
Polymorphism, Restriction Fragment Length
Pregnancy
Risk
Safety
Thrombocytopenia genetics
Thrombocytopenia immunology
Amniotic Fluid cytology
Antigens, Human Platelet immunology
Blood Grouping and Crossmatching
DNA genetics
Fetal Blood chemistry
Isoantibodies blood
Polymerase Chain Reaction
Prenatal Diagnosis
Thrombocytopenia prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 0958-7578
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Transfusion medicine (Oxford, England)
- Publication Type :
- Academic Journal
- Accession number :
- 7516786
- Full Text :
- https://doi.org/10.1111/j.1365-3148.1994.tb00238.x