Detection of alginate lyase by activity staining after sodium dodecil sulfate-polyacrylamide gel electrophoresis and subsequent renaturation.

Authors :: Peciña A
Paneque A
Source :: Analytical biochemistry [Anal Biochem] 1994 Feb 15; Vol. 217 (1), pp. 124-7.
Publication Year :: 1994
Abstract: A slab gel electrophoretic method for the study of bacterial alginate lyase has been developed. By incorporating alginate in acrylamide gels, the method is based on renaturation of the enzyme after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, subsequent staining with cetylpyridinium chloride, and quantification of the spot by densitometric scanning. The molecular mass of alginate lyase can be determined from its position in the gel.

Subjects :: Azotobacter enzymology
Bacterial Proteins metabolism
Electrophoresis, Polyacrylamide Gel methods
Kinetics
Polysaccharide-Lyases metabolism
Protein Denaturation
Sensitivity and Specificity
Sodium Dodecyl Sulfate
Staining and Labeling methods
Bacterial Proteins analysis
Polysaccharide-Lyases analysis

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