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A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1994 Apr 11; Vol. 22 (7), pp. 1138-46. - Publication Year :
- 1994
-
Abstract
- We have identified two regions of non-random purine/pyrimidine strand asymmetry that were nearly identical in sequence in the 5' flanking (promoter) regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene and the human MUC1 gene. These regions contain perfect mirror repeat elements, a sequence motif previously found to be associated with the formation of H-DNA conformations. In this report we demonstrate that a single-stranded non-B DNA conformation exists at low pH in supercoiled plasmids containing the similar mirror repeat elements, and that S1 nuclease digestion maps the single-stranded region to the position of the mirror repeats. In addition, we identify a nuclear protein of approximately 27 kD that binds to single-stranded oligonucleotides corresponding to the purine-rich strand of this region, but not to the pyrimidine-rich strands or to double-stranded oligonucleotides with corresponding purine/pyrimidine strand asymmetry.
- Subjects :
- Base Sequence
Binding, Competitive
Cystic Fibrosis Transmembrane Conductance Regulator
DNA, Single-Stranded chemistry
DNA, Single-Stranded metabolism
Humans
Hydrogen-Ion Concentration
Molecular Sequence Data
Mucin-1
Nucleic Acid Conformation
Oligodeoxyribonucleotides chemistry
Purines metabolism
Repetitive Sequences, Nucleic Acid
Single-Strand Specific DNA and RNA Endonucleases metabolism
Trans-Activators metabolism
Tumor Cells, Cultured
Ultraviolet Rays
Cystic Fibrosis genetics
Membrane Glycoproteins genetics
Mucins genetics
Nuclear Proteins metabolism
Oligodeoxyribonucleotides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 22
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 7513081
- Full Text :
- https://doi.org/10.1093/nar/22.7.1138