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Design and construction of rhinovirus chimeras incorporating immunogens from polio, influenza, and human immunodeficiency viruses.
- Source :
-
Virology [Virology] 1994 Feb; Vol. 198 (2), pp. 703-8. - Publication Year :
- 1994
-
Abstract
- This paper describes the design and construction of chimeric human rhinoviruses that contain immunogenic regions from other pathogens as part of their surface coat proteins. Segments encoding the poliovirus 3 Sabin VP1 and VP2 proteins, the influenza hemagglutinin (HA) glycoprotein, and the human immunodeficiency virus gp120 surface and gp41 transmembrane glycoproteins were inserted into a full-length clone of human rhinovirus 14 (HRV14) at regions corresponding to neutralizing immunogenic sites IA (NIm-IA) and II (NIm-II). Of 12 chimeric constructs described, 3 produced viable virus. An HRV14 chimeric virus containing five amino acids of influenza HA (corresponding to 300 A2 of solvent-accessible surface area) had wild-type HRV14 growth characteristics and was neutralized by four of four anti-influenza HA antisera with reciprocal neutralizing titers ranging from 180 to 330. However, antisera raised in two guinea pigs against the HRV14:influenza HA chimera did not show significant neutralization of relevant strains of influenza. These results are the first to demonstrate the feasibility of making viable chimeras of human rhinoviruses displaying heterologous immunogens.
- Subjects :
- Amino Acid Sequence
Antigens, Viral genetics
Antigens, Viral immunology
Capsid biosynthesis
Capsid genetics
Capsid Proteins
Epitopes genetics
Epitopes immunology
HIV Envelope Protein gp120 genetics
HIV Envelope Protein gp120 immunology
HIV Envelope Protein gp41 genetics
HIV Envelope Protein gp41 immunology
Hemagglutinin Glycoproteins, Influenza Virus
Hemagglutinins, Viral genetics
Hemagglutinins, Viral immunology
Models, Molecular
Molecular Sequence Data
Poliovirus genetics
Poliovirus immunology
Protein Engineering
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins immunology
Rhinovirus growth & development
Antigens, Viral biosynthesis
Capsid immunology
Epitopes biosynthesis
Recombinant Fusion Proteins biosynthesis
Rhinovirus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 198
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 7507283
- Full Text :
- https://doi.org/10.1006/viro.1994.1082