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A human telomeric protein.
- Source :
-
Science (New York, N.Y.) [Science] 1995 Dec 08; Vol. 270 (5242), pp. 1663-7. - Publication Year :
- 1995
-
Abstract
- Telomeres are multifunctional elements that shield chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. A major protein component of human telomeres has been identified and cloned. This factor, TRF, contains one Myb-type DNA-binding repeat and an amino-terminal acidic domain. Immunofluorescent labeling shows that TRF specifically colocalizes with telomeric DNA in human interphase cells and is located at chromosome ends during metaphase. The presence of TRF along the telomeric TTAGGG repeat array demonstrates that human telomeres form a specialized nucleoprotein complex.
- Subjects :
- Amino Acid Sequence
Base Sequence
Cell Nucleus chemistry
Cloning, Molecular
DNA-Binding Proteins analysis
DNA-Binding Proteins genetics
DNA-Binding Proteins isolation & purification
HeLa Cells
Humans
Interphase
Metaphase
Molecular Sequence Data
Molecular Weight
Repetitive Sequences, Nucleic Acid
Sequence Alignment
Transfection
DNA-Binding Proteins chemistry
Telomere chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0036-8075
- Volume :
- 270
- Issue :
- 5242
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 7502076
- Full Text :
- https://doi.org/10.1126/science.270.5242.1663