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Exposure of B-lineage lymphoid cells to low energy electromagnetic fields stimulates Lyn kinase.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1995 Nov 17; Vol. 270 (46), pp. 27666-70. - Publication Year :
- 1995
-
Abstract
- Here, we present evidence that exposure of B-lineage lymphoid cells to low energy electromagnetic fields (EMF) stimulates the protein tyrosine kinases Lyn and Syk, results in tyrosine phosphorylation of multiple electrophoretically distinct substrates, and leads to downstream activation of protein kinase C (PKC). EMF exposure enhances protein tyrosine phosphorylation in Syk deficient but not in Lyn-deficient B-lineage lymphoid cells and stimulates Lyn kinase activity in wild-type as well as Syk-deficient B-lineage lymphoid cells. These results indicate that activation of Lyn kinase is sufficient and mandatory for EMF-induced tyrosine phosphorylation in B-lineage lymphoid cells. The PKC activity increases later than the Lyn activity and pretreatment with the PTK inhibitors genistein or herbimycin A abrogates the EMF-induced PKC signal. Thus, stimulation of Lyn is a proximal and mandatory step in EMF-induced activation of PKC in B-lineage lymphoid cells. Our observations prompt the hypothesis that a delicate growth regulatory balance might be altered in B-lineage lymphoid cells by EMF-induced activation of Lyn.
- Subjects :
- Amino Acid Sequence
B-Lymphocytes enzymology
Benzoquinones
Cell Line
Enzyme Inhibitors pharmacology
Enzyme Precursors deficiency
Genistein
Humans
Intracellular Signaling Peptides and Proteins
Isoflavones pharmacology
Lactams, Macrocyclic
Molecular Sequence Data
Myelin Basic Protein metabolism
Peptide Fragments chemistry
Peptide Fragments metabolism
Phosphoproteins isolation & purification
Phosphoproteins metabolism
Phosphotyrosine analysis
Protein Kinase C metabolism
Protein-Tyrosine Kinases antagonists & inhibitors
Protein-Tyrosine Kinases deficiency
Protein-Tyrosine Kinases radiation effects
Quinones pharmacology
Rifabutin analogs & derivatives
Syk Kinase
B-Lymphocytes radiation effects
Electromagnetic Fields
Protein-Tyrosine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 270
- Issue :
- 46
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7499232
- Full Text :
- https://doi.org/10.1074/jbc.270.46.27666