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Conversion of Salmonella typhimurium to L-forms contributes to the maintenance of acquired immunity against murine typhoid.
- Source :
-
Immunology [Immunology] 1995 Oct; Vol. 86 (2), pp. 206-11. - Publication Year :
- 1995
-
Abstract
- Conversion of Salmonella typhimurium to L-forms, both in vitro and in vivo, resulted in the expression of proteins cross-reacting to the mycobacterial 65,000 MW heat-shock protein (hsp). Immunization of C3H/HeJ mice with a protective dose of stable L-form S. typhimurium induced gamma delta T cells in the liver, in accordance with the multiplication of L-form Salmonella in Kupffer cells. The number of gamma delta T cells decreased after the intracellular growth of L-form Salmonella plateaued. Persistance of the L-forms in Kupffer cells, however, allowed hepatic gamma delta T cells to increase within 48 hr of infection with virulent S. typhimurium. Thus, the intrahepatic colonization of L-form Salmonella seems to keep gamma delta T cells on standby, but the emergence of these T cells does not correlate with the expression of L-form hsp. In addition, Kupffer cells colonized by L-forms constitutively synthesized mRNA for interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha). These results suggest that conversion of S. typhimurium to L-forms in phagocytic cells builds up and maintains acquired resistance, conferred by live-cell vaccines of S. typhimurium, against murine typhoid.
- Subjects :
- Animals
Blotting, Western
Chaperonin 60
Chaperonins immunology
Female
Immunization
Kinetics
Kupffer Cells immunology
Liver immunology
Mice
Mice, Inbred C3H
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, gamma-delta
T-Lymphocyte Subsets immunology
Antigens, Bacterial immunology
Bacterial Proteins
L Forms immunology
Salmonella typhimurium immunology
Typhoid Fever immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 86
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7490119