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The carboxyl extensions of two rat ubiquitin fusion proteins are ribosomal proteins S27a and L40.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1995 Oct 13; Vol. 215 (2), pp. 682-90. - Publication Year :
- 1995
-
Abstract
- Two rat recombinant cDNAs were characterized; they encode fusion proteins that have at their NH2 terminus the conserved 76 amino acid ubiquitin and at their carboxyl terminus the extension ribosomal proteins S27a (80 amino acids and a molecular weight of 9,397) or L40 (52 amino acids and a molecular weight of 6,177). The fusion proteins are processed in a reticulocyte lysate to ubiquitin and either S27a or L40. Hybridization of cDNAs to digests of nuclear DNA suggests that there are 14 to 19 copies of the S27a, and 6 to 10 of the L40, genes. The mRNA for ubiquitin-S27a has about 700 nucleotides and ubiquitin-L40 about 650. Ribosomal proteins S27a and L40 contain zinc finger motifs of the C2-C2 variety. S27a and L40 are related to ribosomal proteins from other species.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cell Nucleus metabolism
DNA metabolism
DNA, Complementary chemistry
DNA, Complementary metabolism
Liver metabolism
Molecular Sequence Data
Open Reading Frames
Rats
Recombinant Fusion Proteins biosynthesis
Ribosomal Proteins biosynthesis
Sequence Homology, Amino Acid
Ubiquitins biosynthesis
Zinc Fingers
Recombinant Fusion Proteins chemistry
Ribosomal Proteins chemistry
Ubiquitins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 215
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 7488009
- Full Text :
- https://doi.org/10.1006/bbrc.1995.2518