A method based on the use of methanol as a stabilizing agent to prepare metal-free glyoxalase I and to reconstitute activity by addition of bivalent metal ions.

Subjects

Subjects :

Cations, Divalent
Erythrocytes enzymology
Humans
In Vitro Techniques
Metals
Apoenzymes metabolism
Apoproteins metabolism
Lactoylglutathione Lyase metabolism
Lyases metabolism
Methanol

Details

Language :

English

ISSN :

0302-4369

Volume :

34

Issue :

7

Database :

MEDLINE

Journal :

Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry

Publication Type :

Academic Journal

Accession number :

7445911

Full Text :

https://doi.org/10.3891/acta.chem.scand.34b-0541