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Stimulation of DNA synthesis in calcium-deprived T51B liver cells by the tumor promoters phenobarbital, saccharin, and 12-O-tetradecanoylphorbol-13-acetate.
- Source :
-
Cancer research [Cancer Res] 1980 Dec; Vol. 40 (12), pp. 4541-5. - Publication Year :
- 1980
-
Abstract
- Extracellular calcium deprivation arrested the proliferative development of T51B rat liver cells in late G1 or S phases. These arrested cells initiated DNA synthesis within an hr after addition of calcium or a tumor promoter such as phorbol-12,13-didecanoate, phenobarbital, saccharin, or 12-O-tetradecanoylphorbol-13-acetate. Nonpromoting relatives of 12-O-tetradecanoylphorbol-13-acetate such as phorbol, 4-O-methyl-12-O-tetradecanoylphorbol-13-acetate, and 4 alpha-phorbol-12,13-didecanoate were unable significantly to induce the calcium-deprived cells to initiate DNA synthesis. It is suggested that an ability to elicit a prompt DNA-synthetic response from calcium-deprived cells might be a useful in vitro indicator of tumor-promoting potential.
- Subjects :
- Animals
Cell Cycle drug effects
Cells, Cultured
Cocarcinogenesis
Liver drug effects
Liver metabolism
Phorbol Esters pharmacology
Rats
Stimulation, Chemical
Calcium deficiency
DNA biosynthesis
Phenobarbital pharmacology
Phorbols pharmacology
Saccharin pharmacology
Tetradecanoylphorbol Acetate pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 40
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 7438088