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[Methods for working with semi-thin epoxide sections in histological practice].

Authors :
Kostilenko IuP
Kovalev EV
Source :
Arkhiv anatomii, gistologii i embriologii [Arkh Anat Gistol Embriol] 1978 Dec; Vol. 75 (12), pp. 68-72.
Publication Year :
1978

Abstract

Since ordinary histological laboratories have no special equipment, semi-thin epoxide embedded sections, to perfectly suitable to realize the resolving capacity of the light optic are not widely used in histological practice. However, even in an ordinary histological laboratory it is possible to master the method of their production. A special device is suggested to fix glass knives in the rotational microtome MPC-2. Simultaneously, a number of suggestions and recommendations are given as to certain specificities for obtaining serial semi-thin sections and the method for their staining. Besides, an original method is offered for producing film preparations by means of mild tissue compression in the process of polymerization in epoxide resin. This method allows not only to study some features in the composition of complex three-dimentional objects, but to obtain a series of semi-thin sections from the film preparation to reveal intimal interconnections between certain structural components.

Subjects

Subjects :
Epoxy Resins
Microtomy methods

Details

Language :
Russian
ISSN :
0004-1947
Volume :
75
Issue :
12
Database :
MEDLINE
Journal :
Arkhiv anatomii, gistologii i embriologii
Publication Type :
Academic Journal
Accession number :
742988