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Flow-cytofluorometric determination of active and total rosettes.
- Source :
-
Journal of immunological methods [J Immunol Methods] 1980; Vol. 35 (3-4), pp. 201-11. - Publication Year :
- 1980
-
Abstract
- A flow-cytofluorometric technique is described for determining active and total rosettes formed by sheep red blood cells with human peripheral blood lymphocytes. This technique correlates well with the microscopic determination of active rosettes, when these are defined as three or more erythrocytes per lymphocyte, and with the determination of total rosettes, when these are defined as four or more erythrocytes per lymphocyte. In the small group of tumor patients tested, the observed decrease in capacity to form active rosettes was found to be dependent on the individual sheep red blood cells used, while the levels of total rosettes were not affected by individual SRBC's and were not significantly different from those of normal blood donors. Flow-cytometry is a rapid, reproducible and objective technique, which permits better measurement than microscopy of total and active rosette levels in both normal donors and cancer patients.
- Subjects :
- Animals
Blood Cell Count
Breast Neoplasms immunology
Bronchial Neoplasms immunology
Female
Fluorometry methods
Humans
Kidney Neoplasms immunology
Male
Pleural Neoplasms immunology
Prostatic Neoplasms immunology
Sheep
Stomach Neoplasms immunology
Uterine Cervical Neoplasms immunology
Uterine Neoplasms immunology
Rosette Formation
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 35
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 7400583
- Full Text :
- https://doi.org/10.1016/0022-1759(80)90247-1